肽水解
肽与蛋白质 → 氨基酸：查看反应物、产物、反应条件与来源依据，探索相关转化和合成路径。
- 试剂
- 6 mol dm-3 盐酸
- 条件
- 约 110 °C 加热 24 h；若需游离氨基酸，再进行中和
- 反应类别
- 水解
- 方程式
- peptide + H2O -> amino acids
概览
肽键可通过与盐酸水溶液长时间加热而水解；若需游离氨基酸，还须进行后处理。
转化
- 方程式
- peptide + H2O -> amino acids
- 试剂
- 6 mol dm-3 盐酸
- 反应环境
- 约 110 °C 加热 24 h；若需游离氨基酸，再进行中和
- 反应类别
- 水解
- 机理
- 酰胺水解
- Evidence level
- reviewed chemistry
底物范围
- 适用范围
- Prolonged heating with concentrated aqueous hydrochloric acid hydrolyses peptide bonds to give amino-acid hydrochloride salts before neutralisation.
- 来源说明
- The displayed product equation is a net relationship. Under the stated acidic conditions the immediate products contain protonated amino groups; alkaline hydrolysis is a distinct procedure and gives carboxylate salts until acid work-up.
相关反应
- Peptide formation: Amino acids → Peptides and proteins
Controlled amino-acid coupling forms peptide bonds; the displayed equation is a net relationship rather than a complete experimental procedure.
参考文献
- Organic Chemistry: Amino Acid Analysis of PeptidesJohn McMurry · OpenStax Organic Chemistry · 2023
Supports complete peptide-bond hydrolysis by heating with aqueous 6 M hydrochloric acid at about 110 degrees Celsius for 24 hours.